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In this study, it was first established that larvae of the nematode Echinuria uncinata are found in the species Daphnia magna in the Fergana Valley, Uzbekistan. In the spring and autumn, 11.5% of the daphnia samples were infected larvae nematode. The morphology and morphometric parameters of nematode larvae are presented. To study the development of larvae in the organism of the intermediate host, D. magna infected nematode eggs with food and studied their development at different temperatures. The larvae reached the fourth invasive stage after 24-26 days at a low temperature of 1-15° C, 9-10 days at 20-22° C and 8-9 days at a relatively high temperature of 24-26° C. In this study was studied development of biology of the larvae of the nematode Echinuria uncinata in the organism of the intermediate hosts-daphnia in Uzbekistan.