HLA-DQ Genotyping of Celiac Disease among Syrian patients

Abstract

Background

Celiac disease is an autoimmune enteropathic disease brought about by consuming gluten in people who are genetically predisposed by carrying HLA DQ2 or/and HLA DQ8 genotypes.

Objectives

To evaluate the distribution of human leukocyte antigen (HLA)-DQ2 and -DQ8 and their relative riskwith celiac disease (CeD) in Syrian patients comparedto healthy Syrian controls.

Subjects &Methods

Genotyping for unrelated CeD patients (n = 100) and unrelated matched healthy controls (n = 100) was performed to identify DQ2 and DQ8 alleles by SSP-PCR

Results:

In this case control study 82% of CeD cases and 24.5 % of controls were carriers of HLA DQ2 whereas 18 % and 8.5 %  ofCeD patients and controls respectively carried HLA DQ8.  The highest risk for the development of CD relating to HLA-DQB was found in patients carrying DQ2.5/DQ8 genotype and DQ2.5/DQ2.5 (1/10) and the lowest risk was found in patients with DQ2.2/DQ8 genotype.

Conclusion: 

In Syrian Patients, celiac disease is strongly related to HLA DQ 2 and DQ 8 genotypes and thus these possess a high positive predictive value for the diagnosis of celiac disease. Additionally, our study highlighted the integral part of these genotypes in the pathogenesis of celiac disease.