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Background: The number of positive cases of Covid-19 in Indonesia in March 2020 was 6760 cases with the number of deaths amounting to 590 people, until 20/4/2020 the number of probable patients became 186,330 people, with 16,763 people were suspected patients.The incidence rate that shows a significant increase definitely puts a very heavy burden on hospitals especially referral hospitals. The limited medical personnels had made working hours in the hospital uncertain. The purpose of this study was to determine the risk factors that most influence the occurrence of burnout of health workers at Pasar Minggu Hospital, South Jakarta.Subjects and Method:An observational analytic study with a cross sectional approach had been done with online data from the Pasar Minggu Regional General Hospital, South Jakarta in August 2020 amid the atmosphere of the Covid-19 pandemic. The target population is health workers who have met the inclusion and exclusion criteria. The variables studied were gender, marital status, handling Covid-19 patients, number of working hours, PPE availability, new policies related to Covid handling, type of workandBurnout. The data were analyzed by univariate, bivariate and multivariate analysis.Results:The number of respondents who had burnout was 23 people (51.1%) compared to those who did not feel that they had burnout. All samples said PPE had been provided and 97.8% said about new policy regarding the handling of Covid-19. There is a relationship between the experience of handling Covid-19 patients with the incidence of burnout with p = 0.010; and OR = 7.69 with 95% CI is 1.447-40.906 and between the number of hours worked with the incidence of burnout in p = 0.012; and OR = 4.96 with 95% CI is 1.388-17.711. The variables that most influenced the incidence of burnout were handling Covid-19 patients with p = 0.043 and OR = 6.45 and 95% CI were 1.061-39.256; and the number of hours worked with p = 0.027 and OR = 5.09 and 95% CI is 1.199-21.568.Conclusion:Burnout of health workers increases by caring Covid-19 patients more often, as well as increasing number of hours of worked health workers, which leads to the burnout of health workers accordingly. Reducing the number of hours worked by increasing health workers and improving working conditions are among the solution.