The Evaluation of Lithiasis/Antilithiatic Activity of Oxalate-Degrading Bacteria and Routine Antibiotics

Abstract

Background: Nephrolithiasis is a common urinary tract disease, in addition to the pain and treatment costs, there might be significant complications resulting from the stones. This study aimed to investigate the lithiasis/antilithiatic activity of oxalate-degrading bacteria and routine antibiotics in rats. Male Wistar rats were divided into 3 groups. The rats in the group I received normal rat chow and drinking water (Positive control group). Groups II (Negative control group), and III rats received 1%Ethylene glycol containing diet for 30 days. Also, groups III rats received probiotic. In this study, 6strains of Lactobacillus and 2Bifidobacterium were used. Also, urinary and serum samples were collected and analyzed on 0 and 30th day. Then, Biochemistry and histopathological studies of the kidney, antibiogram, and oxalate degrading ability tests were carried out. The treatment group showed a significant reduction of hyperoxaluria, also, serum creatinine and calcium were decreased. In the treatment with probiotics, much improvement in the histopathological derangement was observed and showed a remarkable effect as histopathological features of the kidney’s tissue reached up to the normal level which was almost similar to the positive control group. Based on the above results it can be concluded that oxalate-degrading probiotics have significant inhibitory effects on calcium oxalate urolithiasis.