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Nanobiotechnology generates tools from nanotechnology which are used to study biological processes. Nowadays, nanoceria or cerium oxide nanoparticles havebeen employed in varied medicinal applications including antibacterial, antioxidant, antidiabetic, anticancer, drug delivery systems etc. They have unusual property to switch between trivalent and tetravalent oxidation states which consider them excellent candidates for other commercial applications also. The present investigation was focused ongreen synthesis of nanoceria or cerium oxide nanoparticles (CeO2 NPs) from Pomegranate(Punica granatum)peel extract which were also characterized by UV Visible, FT-IR spectroscopy, SEM analysis, EDX and X-ray Diffraction Studies. The antioxidant and antibacterial potential of synthesized CeO2 NPswere also evaluated. The results displayed that they expressed good antimicrobial activity against E. coli and also showedimmense antioxidant activity.