In Vitro Cytotoxic Analysis of Solanum Xanthocarpum induced silver nanoparticles on Brine Shrimp Artemia

Abstract

Introduction

In the branch of nanotechnology, the synthesis of nanoparticles and their development play a significant role because of their wide applications. They are used as antimicrobial agents and prevent the development of multidrug resistant bacteria.All parts of Solanum xanthocarpum  plant, including the stem, flowers, fruits and roots have proved to have medicinal properties. This study aimed to evaluate cytotoxic activity  of  silver nanoparticles synthesised using Solanum Xanthocarpum extract on brine shrimp artemia.

Materials and Methods

The cytotoxicity of silver nanoparticles reinforced solanum xanthocarpum fruit extract was assessed using Brine shrimp assay. 6 well ELISA plates were taken and to each plate 6-8 ml of saltwater was added; followed by adding 10 nauplii to each well. Silver nanoparticles reinforced with solanum xanthocarpum fruit extract was added to each well at different concentrations (5 μL, 10 μL, 20 μL, 30 μL, 50 μL) and was then incubated for 24 h. After 24 h, the total number of live and dead nauplii was counted and the mortality rate was checked.

Results

 At 5 µL concentration,there was a death of 10% of nauplii, at 10 and 20 µL there was

a death of 20% and 30% of nauplii, at 40 µL there was a death of 30% of nauplii and at 80 µL there was a death of 40% of nauplii. It was seen that as the concentration increased the cytotoxicity of the nanoparticles increased.

Conclusion

The use of fruits for the synthesis of silver nanoparticles has many advantages such as, ease with which the process can be scaled up, economic viability and to obtain smaller particle size.The use of lower concentration had lower cytotoxic effects.