Association between MMP 13 Gene Polymorphism [rs2252070] and Irreversible Pulpitis- A Case Control Study

Abstract

Microarray is a recently developed simultaneous analysis of expression patterns of thousands of genes. Expression profiling using the microarray technology represents a powerful approach in the study of gene function in human cells and tissues. The aim of this study is to find the association between MMP 13 gene polymorphism [rs2252070]  and irreversible pulpitis.

Materials and methods

The present study was carried out at the Department of Endodontics , Molecular study lab at Saveetha Dental College, Chennai. This pilot study included a total of 100 participants in the 20-40 year age group. The study participants were divided into two groups, Group A (n=50)- Case group ( Patients with irreversible pulpitis) Group B (n=50)- Control group. Genotyping was performed with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) technique.

Results

Data entry and analysis were performed using SPSS version 17.0. The Hardy–Weinberg equilibrium (HWE) was tested in each group using v2-test. MMP13- A>G gene polymorphisms can be considered a significant genetic marker, emphasizing the pivotal role of genetic components in the pathogenesis of irreversible pulpitis .

Conclusion

This study showed the association between MMP-13 gene polymorphism with irreversible pulpitis in the South Indian population. From genotyping, predictions can be made for patients’ genetic predispositions to disease, allowing strategies for care to become more individualized.