An in Vitro Evaluation of Depth of Tubular Penetration of Ah plus and Endosequence Bioceramic Sealer: A Confocal Laser Scanning Microscopic Investigation

Abstract

OBJECTIVE: The present study investigated the depth of penetration of AH Plus and Endosequence Bioceramic sealer by a confocal laser scanning microscopic investigation.

METHOD: 20 Mandibular single rooted premolar sound teeth were selected. Samples were decoronated at cementoenamel junction to achieve a standardized root length of 14mm. Access opening and biomechanical preparation was done till #F3. Samples were divided into 2 groups for obturation as, Group 1-Endosequence Bioceramic sealer, Group 2- AH Plus sealer. The depth of sealer penetration into dentinal tubules was calculated using confocal laser scanning microscope. One way ANOVA and Tukeys test were applied for the pairwise comparison of the depth of sealer penetration between the groups.

RESULTS: There was statistical significant difference between Group 1 and Group 2 at coronal and middle third of root however there was no statistical significant difference between Group 1 and Group 2 at apical third of root. Group 1 showed more depth of penetration than group 2.

CONCLUSION: The depth and consistency of dentinal tubule penetration of sealer cements appears to be influenced by the chemical and physical characteristics of the materials. Endosequence Bioceramic sealers displayed deeper and more consistent penetration.