Preparation of Nanoparticles of Selenium / Zinc Oxide Reductase in an Environmentally Friendly Green Synthesis Method and Studying Their Effect on Pseudomonas Aeruginosa

Abstract

The current study included testing the two nanocomposites (curcumin / zinc oxide Nps) (curcumin / selenium Nps) on Pseudomonas aeruginosa  which  isolated from burn injuries and surgical wounds, after it was diagnosed by manual and biochemical methods. Two nanocomposites, namely (curcumin / zinc oxide Nps) and (curcumin / selenium Nps) were prepared and charecterised by: UV-Visible spectroscopy and Fourier-transform infrared spectroscopy, infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM), and evaluation of the inhibitory efficacy of nanocomposites and free curcumin in addition to several types of antibiotics (gentamycin ,imipenem). It was highly effective against the studied bacteria. The MIC test was performed by broth dilution method, and it was found that the minimum inhibitory concentration of curcumin was 500 µg / ml. And the MIC for  cur / zno NPs was 2000 µg / ml. Whereas, the MIC for cur / se NPs was 250. The results also indicated an increase in the effect of nanocomposites on the bacteria compared to the free curcumin, and this increase resulted from the synergistic action between the two components of the nanocomposite. The cur / zno Nps results were close to those of gentamycin at 8 mg / ml, and the cur / se Nps results were close to those of the imipenem antibiotic for Pseudomonas aeruginosa.