Molecular and Virological Study of Nuclear Co-Localization of High Oncogenic Risk Human Papillomavirus 16/18(E6/E7) Genes and Overexpressed P53 Protein in Tissue From Malignant and benign laryngeal tumors

Abstract

background: Laryngeal tumors have been the most common malignancies and benign neoplasia of the head and neck tumors . Several studies have demonstrated that infection with human papillomavirus   genotypes  especial high oncogenic one  might be related to the pathogenesis and tumors genesis of the head and neck tumors. Recently, many studies have focused on possible role of HPV oncogenes expression and cell suppressor genes in diagnosing of tumor progression

Objects:  To investigate the correlation of nuclear  co-localization of high risk oncogenes HPV16/18 E6 and E7 expressions with overexpression tumor suppressor  gene p53 protein  

Methods: A retrospective study, 157 formalin- fixed, paraffin-embedded (FFPE) including benign and malignant laryngeal tumors tissues blocks were collected. Molecular detection  and genotyping of high risk HPV 16 and 18 were conducted  by Chromogenic in site hybridization (CISH). Also immunohistochemistry (IHC) tests were perform to investigate the proteins expression of P53 gene as well as high HPV oncogenes E6 and E7 in benign and malignant laryngeal tumors.

Results:  The percentage of positive signals of hrHPV16/18 (E6)-IHC of P53 IHC  in relation to co-localization of  P53 IHC over expression in malignant tumors were (14.3%:2) and  benign tumors (polyps and nodules) were (33.3%:1) and (16.7% :1) respectively whereas the percentage of positive signal of  hr HPV16 E7 -IHC in relation to over expression P53 IHC  in malignant tumors  were (50%:1) and (57.1%:8) respectively and in benign tumors (polyps and nodules) were (33.3%:1) and( 100%:1) respectively. In results of hr HPV18(E7) – IHC signal and co-localization with 50%:1 and benign tumors  (polyps and nodules) were (16.7%:1) for each one. The results of our study show the highest mean value of age was in malignant  groups (56.91±17.12), while the lowest mean age was at control groups (27.25±12.15). the mean age of male (43.43±19.91)  was more than female (39.68±19.25).

Conclusion: depending of IHC results of  both hrHPV16/18 (E6) and (E7) gene expression  as well as P53 over expression revealed that high oncogenic genotypes may contribute in pathogenesis and carcinogenesis of early  steps of benign  and malignant tumors