Different Effects of Black Rice Extract Supplementation on PRMT-1 Mrna Expression and DDAH-1 Mrna Expression in NZW Rabbits Fed a High-Fat Diet.
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Abstract
Scope: Previous investigations demonstrated that black rice extract reduces or retards endothelial dysfunction in hypercholesterolemic rabbits. However, the effects of this extract remain unclear. This study assessed the effect of black rice extract supplementation on the serum concentrations of oxidized LDL and asymmetric dimethylarginine (ADMA) and the mRNA expression of protein methyltransferase-1 (PRMT-1) and dimethylarginine dimethylaminohydrolase-1 (DDAH-1) in New Zealand white (NZW) rabbits fed a high-fat diet. Methods and results: In this randomized controlled trial, 27 NZW rabbits were randomly divided into three groups that received a normal diet, a high-fat (normal diet + 5% lard and 12% egg yolk) diet, or a high-fat diet supplemented with 0.6 g/100 g black rice extract for 4 weeks. Black rice extract supplementation for 4 weeks significantly decreased the serum concentrations of total cholesterol, F2-isoprostanes, oxidized LDL, and ADMA, decreased the mRNA expression of PRMT-1 and increased the mRNA expression of DDAH-1 (p<0.05).
Conclusion: Black rice extract supplementation attenuates LDL oxidation, downregulates PRMT-1 mRNA expression, upregulates DDAH-1 mRNA expression, and inhibits the increase in ADMA concentration in rabbits fed a high-fat diet