Carbapenem Resistant Klebsiella Pneumonia (CRKP) Harbor NDM-1 Gene Isolated from Wastewaters in Basrah, Iraq

Abstract

Resistance to carbapenems is developing around the world Klebsiella pneumonia resistance to carbapenems is associated with production of carbapenem-hydrolyzing class B metallo-β-lactamases (MBL).New Delhi metallo-β-lactamase 1 (NDM-1) is one of the most recently discovered MBL among E.coli .The present work was aimed to study the prevalence of bla NDM-1 gene among carbapenem-resistanti isolates from Klebsiella pneumonia five s Wastewaters in Basrah, Iraq Isolates identified and antibiotics susceptibility were assayed by using VITEK2Compact.Phenotypic detection of carbapenemase was performed using combined-disc synergy test (CDST), Chrom ID Carba agar and using RAPIDEC CARBA NP test, isolates were also subjected to the polymerase chain reaction detection of bla NDM-1 gene.