The effect of Green Tea Extract, Black Tea exctract and Caffeine to the Length of Femur on the DDY Strain Male Mice Which Given Calcium

Abstract

The purpose of this study was to assess the effect of some kind of natural ingredients that are commonly consumed to the length of the femur. The substances tested were green tea, black tea and caffeine, in DDY strain male mice were given calcium. The target of this study was to prove information about inhibition of bone growth from the length of green tea, black tea and caffeine. The experimental laboratory study used 32 samples of mice devided  into 8 groups. The subject was treated to compare the effects of caffeine, green tea, and black tea on the growth of the femur length. The data obtained were analyzed using one way ANOVA and Duncan's post hoc test, with a confidence level of 95%. The results showed significant differences in bone length from each treatment group with a value of p=0.000 (p<0.05). The longest bones were shown by a group given only calcium (15.65 ± 0.26 mm), while the shortest was shown by a group given only black tea (13.68 ± 0.13 mm). The groups, which were given green tea and caffeine, showed significantly different bone lengths with negative control, but their effectiveness was not comparable to those given only calcium. The group, which given black tea, showed the same bone length as the negative control group although it was administered simultaneously with calcium. The conclusion of this study, among the greens, the black and caffeine, the inhibitory effect of long growth of the mice femur, that was given calcium, was shown by the group given black tea.