The Molecular Activity of Gingerol on Inhibits Proliferation of Breast Cancer Cell Line (MCF7) Through Caspase Activity

Abstract

This study was conducted to determine the role and molecular function of phenolic gingerol in regulating the cell proliferation of MCF-7 breast cancer cells, a compound isolated from Zingiberofficinale. Different gingerol concentrations from (1-1000) μg/ml were prepared to assess gingerol's cytotoxic effect on MCF-7 cell lines, cell line exposure periods were measured in (24) hours.Under complete sterile conditions on a microtitration plate. Results showed that, after exposure to cell line for 24 hours, Cells treated with gingerol showed higher cell-death percentagethan untreated cells as quantified bythe MTT assay, with a 100 μg/mL inhibition rate of MCF-7 of 83.2 percent following 24 hour of exposure.The capacity of gingerol to induce apoptosis to an infected cell under a fluorescence microscope has been studied by crystal violet stain to measure the percentage of induction of apoptosis. A significant increase of the expression of caspase-3, caspase-8 and caspase-9 in comparison with untreated cells has revealed the gingerol effect on the MCF-7 line. The role of apoptosis, its high potential as an antitumor agent, was demonstrated in this study.