Stereotyping and Genotype of Poliovirus Aetiology of Acute Flaccid Paralysis by Using Intratypic Differentiation in Iraq.

Abstract

The current study aims to determine the causes of Acute Flaccid Paralysis AFP cases for ages less than 15 years. Four hundred eighty stool specimens were collected during the period from January to December 2019 and received to  National Polio Laboratory ( NPL\ IRAQ)  that is  responsible for the laboratory part of AFP surveillance project specimens collected from all Iraqi provinces.

The results showed that 65 (13.5%) sample from total samples are non –polio  viruses that  have  specific cytopathic effects  (CPE ) on cell culture  in  RD cells only, while 11 ( 2.3 %) samples were positive  for polio virus which isolated from AFP cases, all these cases showed  CPE  on L20B cells only. All these samples were from patient vaccinated with the type of OPV in varying number of orally bivalent oral polio vaccine (bOPV ) dose. The results also showed that 404 (84.2%) that were (Negative) no appears CPE on both cells.

All  positive  Poliovirus  cases 11 (16.9%)  were  serotyping   by use  rt qPCR method  ,the  current study confirmed  that  Sabin like Poliovirus  serotype  three (SLPV3)  7 cases  (  63.6%) from all  positive  Poliovirus  cases   followed by Sabin like Poliovirus  serotype  one  (SLPV1   )  3 cases  ( 27.2%), while this study showed one case 1 (9%) Sabin like Poliovirus  serotype  one  (SLPV1   )   is  negative  (discordant)  in ITD method. The results also showed no Wild polio virus  or Vaccine derived poliovirus (VDPV) isolates.