Isolation, Characterization and Identification of Molecular Lactic Acid is Isolated from Bilih Fish (Mystacoleucuspadangensis) Lake Singkarak Potential as a Probiotic

Abstract

The Singkarak lake bilih fish (Mystacoleuseuspadangensis) is a fish originating from the Singkarak lake of West Sumatra Province. This study aims to obtain lactic acid bacterial candidate isolates from probiotic candidates from BilihDanauSingkarak fish which have not been utilized and have great potential to be developed. Each species of lactic acid bacteria has different probiotic effects, so selection and identification is needed to get a good probiotic strain. This research is a descriptive study and laboratory analysis to determine for sure the lactic acid bacteria found in bilih fish. LAB was isolated selectively using de Man Rogosa Sharpe Agar media. Selection was carried out by morphological observations and gram staining. Furthermore, testing the biochemical properties and identification of lactic acid bacteria in this study using the molecular method lderuiflcarlon with the 16s rRNA marker gene. The results of the isolation of lactic acid bacteria obtained the best IB1 isolates which were Gram-positive, Bacilli, catalase negative, homofermentative bacteria with the results of screening probiotic candidates having inhibitory power against pathogenic bacteria Escherichia coli O157 27.29 mm, resistance to bile salts, viability of lactic acid bacteria 77%, and The pH of the stomach, the viability of lactic acid bacteria was 30.56%, and the type of Lactobasillus fermentum was obtained. with a length of 1492 bp base.