Characterization of Bacteria from Different Street Foods and Their Antibiotic Resistance Profile from different Selected Area in Gazipur, Bangladesh

Abstract

Bacterial contamination of retorted foods has become an important public health issue that foods is sold by street vendors. In Bangladesh, forborne illness is very commonn and is mainly occurred by consumptions of contaminated street foods. This study was conducted to bend the microbiological standard of various types of street foods (chotpoti and fuchka) sold by street vendors of different areas at Gazipur in Bangladesh. Total 32 food samples from 2 different types as chotpoti and fuchka were collected from different areas. Bacterial isolation, identication, colony count and antibiotic sensitivity testing were performed by using the succeeding level of bacteriological techniques. Total viable count of bacteria is calculated with CFU/g units range from 9*105 CFU/g to 5.3*107CFU/g. For enumeration and identification of test organisms all food samples were inoculated into different types of selective media including Eosin Methylene Blue agar (EMB), Salmonella- Shigella agar (SS) and Mannitol salt agar (MS). From different street foods at gazipur district we isolate the highest frequent of E. coli 15(37.5%) succeeding by Staphylococcus spp 10 (25%), Klebsiella spp 8(20%), Salmonella spp 5(12.5%) and Shigella spp 2(5%). E. coli isolates were resistant to ampicillin, penecilin G, chloramphenicol and cephalexin where sensitive to gentamicin and ciprofloxacin. Salmonella spp were sensitive only ciprofloxacin and remaining antibiotic disc were resistant. In case of Shigella spp gentamicin and ciprofloxacin were sensitive where remaining disc were resistant.  Klebsiella spp. isolates were resistant to ampicillin, cephalexin and sensitive to gentamicin, neomycin and ciprofloxacin. Gram positive Staphylococcus spp. were resistant to ampicillin, cephalexin and vankomycin and sensitive to were resistant to ampicillin, vancomycin, and cephalexin and sensitive to gentamicin and ciprofloxacin. Ciprofloxacin shown 100% sensitivity and cephalexin shown 100% resistant in all tested bacterial isolates. This stud y confirmed multidrug resistance bacteria from different local street foods in Gazipur City that causes foodborne illness and impact on public health hazard. Recommendation of this study are proper handling of forxl, training on safety practices and regular inspection..