Impact of Silver Nanoparticles on Hematological Profiles and Hepatorenal Functions in Photosensitivity :In Vivo

Abstract

Silver nanoparticles (AgNPs) play an essential role in nanomedicine . In the current study, the impact of AgNPs was investigated in rats with photosensitivity induced by administration of Hypericum perforatum (HP). A total of 36 male rats were assigned randomly into 4 groups of 9 rats each. Control group (healthy), positive control group (HP induced photosensitivity), HP+AgNPs group, and AgNPs group (positive treated only with AgNPs). A wide range of blood parameters, serum biomarkers of hepatic and renal functions, anti-oxidants enzymatic activity, pro-inflammatory markers were measured. Hematological results showed that AgNPs caused a significant increase in hemoglobin (Hb), packed cell volume (PCV), and mean corpuscular volume (MCV) with reducing of white blood cells (WBCs) down to normal level following 15 days of AgNPs treatment in photosensitized rats compared to positive control group. Interestingly, AgNPs enhanced hepatorenal functions through ameliorating effect on the serum markers of hepatic and renal function at 15 days of treatment compared to positive control group. In addition, they markedly reduced the oxidative stress with obvious free radical scavenging activity at 15 days of treatment compared to positive control group. Also, results showed a significant decrease in pro inflammatory cytokines in AgNPs treated photosensitized rats, compared to positive control group. In brief, these finding would provide a new insight into the role of AgNPs that enhances the immunological, hematological, enzymatic antioxidant activity, and liver and kidney functions in photosensitized rats, which potentially could be beneficial to amend the clinical results of photosensitivity.