Positive effect of Allium sativum and Citrus aurantifolium plants on glucose level in diabetic rats using Alloxan

Abstract

  The current study was  designated to investigate the effect of Allium sativum and Citrus aurantifolium plants (C. aurantifolium ) extract on blood glucose and  lipid profile as well as body weight in diabetic rats, thirty adult rats  were divided into five groups (6rat for each). G1 :served as healthy control given tap water G2 :diabetic without treatment , G3: diabetic treated with garlic extract 150 mg\kg body weight, G4:diabetic treated with C. aurantifolium and G5: group treated with150 mg\kg mix extracts (garlic and C. aurantifolium).All treated animals were given orally 1 ml of extracts every day. The effects of aqueous extract  of garlic and C. aurantifolium  on some  parameters were investigated in normal and diabetic rats  such as fasting blood sugar(FBS), fasting serum insulin(F.serum insulin) ,ketone body , lipid profile (Cholesterol , Triglyceride, High density lipoprotein( HDL),and Low density lipoprotein LDL) , the results investigated  a significant decrease in glucose levels in group treated with mix plants(159.73±2.67) as compared with  garlic (176.10±2.69) and C. aurantifolium (205.45±4.09) after twenty days of treatment, also the results display a significant improvement in insulin level in groups which treated with garlic , C. aurantifolium and mix plants (4.61±1.22 ,3.69±1.03  and 4.21±1.50 mg\dl respectively)  compared with untreated  diabetic group ( 1.88±0. 60), the value of ketone body was decreased in all treated groups  with garlic, C. aurantifolium and mix plants(5.73±1.98, 6.94±2.86 and4.90±1.84) respectively,  the results  report a significant decline in cholesterol level in groups treated with garlic ,C. aurantifolium  and mix extract (91±3.98, 95±4.35 and 87±2.45 )mg\dl respectively compared with the untreated diabetic group (108±3.08 mg\d). the level of Triglyceride reported a significant p<0.05 decrease   in all groups treated with garlic ,C. aurantifolium  and mix extract ( 89±3.96, 90±3.78  and  85±3.73) mg/dl respectively compared with untreated diabetic group (102±4.43) mg\dl . HDL level revealed significant p<0.05  improvement in groups treated with garlic ,C. aurantifolium  and mix extract(38±2.94, 32±2.09 and 42±3.02) mg\dl compared with control  and untreated diabetic groups (30±2.12 and 24±3.53) mg\dl , reduced level of LDL were assessed  in groups treated with garlic ,C. aurantifolium  and mix extract(40±3.59, 43±4.50 and30±3.42) mg\dl.  There was a significant p<0.05 improvement in body weight in the group treated with mix plants extract (184 ±4.30 g) after  30 days as compared with these groups at the beginning of experiment (178 ±2.93) g.