Study of Dental Plaque Isolates, Their Properties of Biofilm Formation and Susceptibility to Antibiotics

Abstract

The aim of this study is to determine the microorganisms’ profile of the supragingival dental plaque with their properties of biofilm formation, to test the antibiotics resistance ability and to reviewing the role of C. albicans in caries using an in vitro dual-species cariogenic oral biofilm model. The target microorganisms were isolated from attached plaque of the teeth from the patients attending Dental Clinic, Health Clinic Centre Mosul University. The ability of formation biofilm using different surfaces were tested. The antibiotic susceptibility and an in vitro dual-species cariogenic oral biofilm model were conducted. A total of 39 isolates were effectively acquired. To screen the selected isolates according to its biofilm formation ability, three methods were used for that. The results showed that almost all the isolates can form visible growing biofilms. Antibiotic susceptibility assays results showed that most of the isolates were resistant to a wide range of antibiotics.The pH values of dual-species biofilms after 48 h of growth, were above the critical demineralization zone of pH 5.3–5.5. It was concluded that almost all the isolates from patients attending Dental Clinic, can form visible growing biofilms. Further, the study revealed that antimicrobial resistance of isolates for the patients with dental caries conditions is associated with biofilm formation.Our finding suggests that C. albicans are not a microorganism of cariogenesis, it is by actively increasing pH, could prevent caries.