Anti-inflammatory and Anti-oxidative Effects of Vitamin D Supplementation in Type 2 Diabetic Rats’ Model

Abstract

Objectives: The study aimed to assess the anti-inflammatory and anti-oxidative effects of vitamin D supplementation to type 2 diabetic rats.

Subjects and methods: Sixty male Albino Wistar mature rats, weighing 250±10 g were used. Normal control group: 10 rats kept on regular diet and received no treatment. Normal control group+100IU vitamin D: 5 rats kept on regular diet with 1000IU vitamin D supplementation. Diabetes was induced in the rest of animals (45 rats) by single dose of streptozotocin and fed high fat diet, then divided into 3 equal groups. Diabetic group: 15 diabetic rats received no treatment. Diabetic group+100IU vitamin D: 15 diabetic rats supplemented with 1000IU vitamin D. Diabetic group+200IU vitamin D: 15 diabetic rats supplemented with 2000IU vitamin D.

Results: 1000 and 2000IU vitamin D administration to diabetic rats significantly reduced the inflammatory markers TNF-α and NFkB to near the normal control values. 1000IU vitamin D supplementation was associated with a significant improvement in oxidant markers in diabetic rats with significant decrease of MDA level (p= 0.009), and non-significant increase of GSH level, but with higher level of SOD (p= 0.028). While 2000IU dose has led to significantly elevated GSH (p= 0.003) and SOD (p= 0.003) and lowered MDA level (p= 0.003). Low and high vitamin D regimen to diabetic rats induce improvement of the histopathological changes in the architecture of pancreas, liver and skeletal muscles.

Conclusion: Supplementations with 1000 to 2000IU vitamin D to diabetic rats significantly reduced the inflammatory markers to near normal values, improved anti-oxidant markers, and showed protective effects on the pancreas, liver and skeletal muscles.