Molecular profile of mecA, tst-1, Hla, Hlb, eta,etb,Erma, and Ermb virulence genes in Staphylococcus aureus using RAPD - PCR .
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Abstract
The present work highlights the molecular typing of thirty Staphylococcus aureus clinical strains isolated from skin lesions of patients, consulted a dermatologist at Diwaniyah Teaching Hospital in Iraq, using RAPD – PCR to detect of eight virulence genes (mecA, tst-1, Hla, Hlb, eta,etb, Erma, and Ermb). Tested strains gave the profile of St.aureus, deduced from yellow colonies on mannitol salt agar , and oxacillin (methicillin) resistance( MRSA). The occurrence of virulence genes eta, etb, Hlb, Erma, and Ermb among the thirty pathogenic Staph.aureus were 16.66 , 50 ,60, 16.66 and 26.66% ,respectively. The RAPD-640 PCR could categorize the thirty clinical strains under study into 19 groups strains (19 clades), regarding the obtained DNA banding profile in each profile. The present data would underpin that Staph.aureus MRSA is the most predominate pathogen among patients with skin lesions .