Cytochrome Oxidase Subunit I (COI) Gene Sequencing for Identification of Cephalopina Titillator local Isolates from Camels in Thi-Qar Province/Iraq

Abstract

This study was conducted for the period from the first of the month of February 2020 to 30 of October 2020 in the Thi-QarProvince ,A twenty DNA samples were used to detects the parasite by using specific DNA primer of cytochrome oxidase gene COI  which amplify 545 bp PCR products for Cephalopina titillator, then the positive products were subjected to DNA sequencing and the result was analyze by (MEGA X version) and NCBI-BLAST programs. The results of the PCR showed the positive identity of the C. titillator in the samples in agarose gel electrophoresis with in 545bp for the gene specific regions of the C. titillator, the DNA sequences appear the presence of a new strain of parasite under study that recorded in NCBI gene bank under IQ 1 (ACCESSION: MW167083). We can conclude that the Cephalopeniatitillotarhas a new strain recorded in Iraqi isolates called Cephalopina titillator isolate IQ1 cytochrome c oxidase subunit I version (MW167083.1) and mitochondrial COX I gene is a powerful genetic marker for phylogenetic studies.