Phytochemical Screening by FTIR Spectroscopic Analysis and Anti-Bacterial Activity of Methanolic Extract of Selected Medicinal Plant of Anethum Graveolens and Plantago Major

Abstract

The objectives of this study were analysis of the secondary metabolite products of of Anethum graveolens and Plantago major and evaluation of anti-bacterial activity. The FTIR analysis of Anethum graveolens proved the presence of functional  assignment Alkenes, Alkyl halides,  Amide, and Alkane with Peak (Wave number cm-ˡ) 667.3 Bending  (Strong =C–H Bond), 758.0 Bending  (Strong =C–H Bond), 1026.1 Stretch (Strong C-F Bond), 1238.6 Stretch  (Strong C-F Bond), 1317.2 Stretch  (Strong C-F Bond), 1375.2 Stretch  (Strong C-F Bond), 1608.5 Stretch (Strong N-H Bond), 1732.1 Stretch (Strong C=O Bond), 2850.4 Stretch (Strong C-H Bond), 2920.9 Stretch (Strong C–H Bond), The FTIR analysis of Plantago major proved the presence of functional  assignment Alkenes, Alkyl halides,  Amide, and Alkane with Peak (Wave number cm-ˡ) 1001.06 Stretch (Strong C-F Bond), 1049.28 Stretch (Strong C-F Bond), 1238.30 Stretch (Strong C-F Bond), 1386.82 Stretch  (Strong C-F Bond), 1394.53 Stretch  (Strong C-F Bond), 1517.98 Stretch  (Medium C=C Bond), 1635.64 Stretch (Bending N-H Bond), 2854.65 Stretch (Strong C-H Bond), 2926.01 Stretch (Strong C-H Bond). Zone of inhibition (mm) of test bacterial strains to Anethum graveolens bioactive compounds and standard antibiotics were (5.001±0.31), (4.006±0.23), (4.961±0.21), and (3.996±0.20) uses Anethum graveolens bioactive compounds, and (2.103±0.11), (2.431±0.11), (2.011±0.11), and (1.027±0.01) uses Rifambin, and (1.732±0.10), (2.009±0.11), (1.417±0.10), and (1.915±0.11) uses Kanamycin for Staphylococcus aureus, Escherichia coli, Proteus mirabilis, Klebsiella pneumonia respectively. Zone of inhibition (mm) of test bacterial strains to Plantago major bioactive compounds and standard antibiotics were (4.591±0.21), (5.014±0.30), (4.992±0.30), and (4.087±0.21) uses Plantago major bioactive compounds, and (3.811±0.21), (3.001±0.19), (1.961±0.12), and (1.063±0.10) uses Rifambin, and (2.007±0.10), (2.038±0.11), (2.001±0.10), and (2.075±0.11) uses Kanamycin for Staphylococcus aureus, Escherichia coli, Proteus mirabilis, Klebsiella pneumonia respectively.