Antimicrobal Activity of Ethnotraditional Herb Extracts against Coagulase-Negative Staphylococci (Cns) Isolated From Dairy Cows with Mastitis in Lopburi Province, Thailand

Abstract

This study aimed to investigate the efficacy of traditional herb extracts on inhibition of coagulase-negative staphylococci (CNS) isolated from dairy cows with mastitis. Milk samples were collected and diagnosed subclinical mastitis by California mastitis test (CMT), then followed by CNSisolation and identification. Leaves herbal plants, Syzygium cumini, Millingtonia hortensis, and Zizyphus mauritiana were extracted with water or 95% methanol. Crude extracts were analyzed phytochemical compounds and determined the effectiveness of CNS inhibition using the disc diffusion technique, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Seventeen isolates of CNS were recovered. Aqueous extracts of S. cumini and methanol extracts of Z. mauritiana showed the most pronounced inhibition zone on the tested CNS isolates, with a range of 14.3 to 21.0 mm and 14.0 to 20.0 mm, respectively. The MIC and MBC of all extracts ranged from 1.56 to 12.5 mg/ml. However, the aqueous extracts of S. cumini leaves and the methanol extracts of Z. mauritiana leaves showed strong antimicrobial effects (1.56 mg/ml) against CNS isolated from bovine mastitis milk samples. The ethnotraditional herb extracts of S. cumini and Z. mauritiana leaf could be effectively used against bovine mastitis CNS bacteria and their antibacterial activity were confirmed by phytochemical compounds presence.