The Effect of Intralesional Injection of Salbutamol in Experimentally Induced Hypertrophic Scar
Main Article Content
Abstract
Background: Hypertrophic scars are developed as a result of exaggerated dermal tissue proliferation after skin injury .Althoughmany theories regarding generation of hypertrophic scar exist; however the precise pathophysiological process remain unclear. Many various treatment modalities have been implicated in their treatment, however currently there is no satisfactory strategy for treating all keloid lesions; moreover the standard intralesional injection of steroid are associated with a lot of local side effects and there is need for search for other treatment options.
Aim of the study: to evaluate the role of intralesional effect of salbutamol in experimentally induced hypertrophic scar in rabbits.
Materials and methods:
Forty healthy male albino rabbits between 10 and 12 months of age were used in the study. The animals were left for 2 days to acclimatize to the animal house conditions of controlled temperature (28-30ºC), allowed free access to water ad libitum and pellet . Forty rabbits were divided into five groups, ten rabbits pergroup .
Group1. Healthy animal group.
Group2. Hypertrophic scar was induced and the animals left without treatment.
Group3. Hypertrophic scar was induced then the animals treated with intralesional triamcinolone acetonide (standard drug) once weekly for 4weeks.
Group4. Hypertrophic scar was induced scar then the animals treated with intralesional salbutamol 0.2% (investigated drug ) once weekly for 4weeks.
The results measures included evaluation of histopathology of dermal sections, transforming growth factor beta 1 level (TGF-β1) , and collagen IIIin skin of rabbit’0s ears .
Results
By comparison with the control group, intralesional administration of salbutamol significantly reduce the scars in ear of the rabbit models , this effect was demonstrated on the basis of histological and biochemical parameters , moreover this therapeutic effect was comparable to standard intralesional steroid injection.
Conclusion
Intralesional salbutamol injection has powerful therapeutic effects on deposition of collagen, fibroblasts hyperplasia , and micro angiogenesis in scars on rabbit ears without demonstration a local side effects.