Association of IL-4 −590 (C>T) Gene polymorphism with the Levels of Serum IL-4 and IgE on the Risk of Bronchial Asthma in Babylon Province/Iraq

Abstract

Seventy-five bronchial asthma patients and fifty apparently healthy as a control group. The result of this study showed a highly significant (P≤ 0.01) elevation level of the Total IgE in the serum of asthma between patients and control, (456.45 ± 290.106 vs. 30.08 ± 24.414) respectively. The result shows a highly significant elevation differences in the level of the IL-4 in the patients and control (at P -value ≤ 0.01), (445.1978 ± 356.3931vs 54.4255 ± 19.80227) pg./ml respectively.  The DNA was extracted from blood samples. Detection of genotype IL-4 SNP -590 were achieved by ARMS-PCR. The results revealed significant differences in the frequency distributions of the of heterozygous CT among case and control (0.019) at P<0.05, the result show protective role, (O. R: 0.4186; C.I.95%:0.19 – 0.88). Although there were no significant differences in wild homozygous (CC) and mutant homozygous (TT) polymorphisms between case and control, the result show rick role with asthmatic among individuals, (O. R: 1.6; C.I.95%:0.74 – 3.53) and (O. R: 2.441; C.I.95%:0.87 – 6.39) respectively, it’s may include asthmatic predisposition factors in Iraqi patients. The present study shows that the presence of IgE, and IL-4 serum levels were significantly associated with genotype of IL4-590 (CC, TC, TT) among patients and control at (P ≤ 0.05).