The Protective Effect of Aqueous of Hylocereus Polyrhizus Fruits on some Histological Changes of the Testes of Treated Male Rats Carbon Tetrachloride

Abstract

This study aimed to investigate the effect of Hylocereus polyrhizus fruits in protecting testis tissue from damage caused by carbon tetrachloride toxicity. In this research, it was suggested that the aqueous extract of red dragon fruit could positively affect the testicular tissue, and to verify this, (24) adult male laboratory rats were divided, weight ranged between (250 - 310) grams and their ages between (11- 13) approximately one week randomly into four groups with (6) animals for each group as follows: the first (negative) control group, the second group, the positive group, were injected with carbon tetrachloride at a concentration of 0.1 ml / 100 g of body weight under the peritoneum and for two days per week, the third group animals I was dosed with aqueous extract of dragon fruits at a concentration of (650) mg / kg of body weight by mouth daily throughout the trial period. The fourth group of animals were dosed with aqueous extract of Red dragon fruits at a concentration of (650) mg / kg by mouth daily an hour before they were injected with carbon tetrachloride at a concentration 0.1 ml / 100 g, for 2 days a week. After 30 days of the experiment, a microscopic examination of the testicular tissue was performed. In the positive group, the presence of interstitial spaces between the seminiferous tubules, the decrease in the size of the germinal epithelial cell layer, fewer Leydig cells, absence of sperm in the lumen, the increase in the diameter of the lumen, the necrosis of the cells lining the tubules and the decrease in the diameters of the seminiferous tubules as well as a decrease in The level of testosterone, luteinizing hormone (LH) and (FSH) when compared with the control group. In the third group, the normal testis structure with the seminiferous tubules appears, increase in the number of epithelial germ layer and sperms, a decrease in the lumen, with the presence of your Leydig cells in the interstitial tissue and an increase in the number of germ cell layers. The formation of the sperm when compared with the control group and the fourth group shows the normal testes structure with the seminiferous tubules, as well as an increase in the number of sperms and a decrease in the lumen with the presence of your Leydig cells in the interstitial tissue, an increase in the number of the germinal epithelial cell layer, an increase in the hormone testosterone and the (LH) and (FSH) when compared to a group of carbon tetrachloride, we conclude from this that the aqueous extract of Hylocereus polyrhizus fruits has a positive effect in the process of improving Fertility in male rats is also highly effective in antioxidants and reduces the histological changes caused by carbon tetrachloride.