Influence of Foliar Spraying with Potassium, Zinc, and Copper Nano-fertilizers on Some Vegetative Growth Characteristics and Anthocyanin Gene Expression of Pomegranate Transplants

Abstract

This trial was carried out during the growing season of 2018/2019 to study the effect of nano-potassium, nano-zinc and nano-copper levels (0,1,2 g.L-1 ), (0,2,3 g.L-1) and (0,0.5,1 g.L-1) sprayed twice(15/3/2019 and 15/4/2019). The experiment was designed using R.C.B.D with three replicates. Results exhibited that the nano-potassium affected in Diameter increased, Leaves numbers increased/transplant, Leaves area, Total chlorophyll, P %, and K %. the nano-zinc affected Total chlorophyll for both concentrations reached 69.833 and 73.211100mg/g fresh weight, while nitrogen was highly significant when pomegranates transplant treated with nano-zinc by both concentrations 2 and 3 g.L^-1reached 1.95 and 1.97%. The percentage of zinc appeared high significant when pomegranate treated with nano-zinc for both concentrations (2 and 3 g.L^-1) reached 22.3 and 22.7 mg.Kg^-1 respectively. The high percentage of copper when pomegranate transplant treated with nano-copper (0.5 and 1 g.L^-1) reached 8.9 and 9.1 mg.Kg^-1 respectively. The expression of ANSpg gene was the best treatment when pomegranate transplant treated with potassium with both concentrations compared with control.