Comparative Study on Phytochemical, Antioxidant and Anticancer Properties of Centellaasiatica and Their Impact on P53 Expression in MCF-7 Cells

Abstract

Centellaasiatica (Linn.) is traditionally used as medicine from ancient days and reported for its wide therapeutic properties. It is widely used for leprosy, lupus, varicose ulcers, eczema, psoriasis, diarrhoea, fever, amenorrhea, anxiety etc. Thus present study had done on screening of phytochemicals, determination of antioxidant potential and their anticancer properties of two different traits in Centellaasiatica (C₁and C₂). The preliminary screening of phytochemicals by ethanol, chloroform and aqueous extract revealed a broad spectrum of secondary metabolites. Among the extracts ethanol extract contains rich in flavonoid content and it exhibited potential antioxidant activity at a concentration of 100 mg/mL 63.41% in C1 and 65.21% in C2. The anticancer study showed IC50of 187.78µg mL for C1 and 86.11 µg mL for C2 against MCF-7. The extract against L929 showed no cytotoxic effect towards normal cells with 698.55 µg/mL.  Further analysis on non-toxic behaviour showed ethanol extracts of C2 is less toxic than C₁. The test compound namely C₂ showing p53 expression at 63.40 in terms of mean fluorescence intensity (MFI). The present study affirms that the leaf extracts of two different traits of Centellaasiatica has enormous potential and effective as natural antioxidant and anticancer. The phytochemicals present may responsible for antioxidant and other pharmacological effects of the plants. Based on the results it can be concluded that the C2 ethanol extracts holds significant antioxidant and anticancer properties.