Comparison of Mrt-PCR Method with Microscopy for Giardialamblia Diagnosis

Abstract

The current study was aimed to determine G. lambliaby multiplex real-time polymerase chain reaction (mRT-PCR) in faeces samples of children diagnosed by microscopic method. This study was conducted between April and November 2019 in Kirkuk, Iraq. The study population includes 283 children aged 0-12 years visiting Children's Hospital and Azadi Teaching Hospital with diarrhea. The faeceswere first analyzed by using microscopic method followed by concentration of faecessamples for the presence of G. lamblia. The results show that the overall positive ratio of Giardia infection among children below 12 years was determined by microscopy in 7.4% (21/283). After performing mReal Time-PCR analysis, the finding showed 24(8.5%) were positive for G. lamblia, which were negative under microscopic examination, while 21 microscopic diagnostic suspected positive cases were again diagnosed by mRT-PCR and found positive for only five samples.