Differential detection and phylogenic study of Entamoeba spp in Rats

Abstract

Rats  are found worldwide and live close to human inhabitation. Rats are reservoir hosts for human amoebiasis. The present study  microscopic examination show that 14% of  fecal samples in rats , the highest rate of infection (15.5%) in Alshaeb , whereas the lowest rate (12.5) was recorded in AbuGharib , the study showed no significant differences .The study recorded  a significant differences (p<0.01) in percentage of infected rats during different months ,there was no significant differences recorded between infected males and females . The study revealed (16.4%)  in males and (9.09%) in females of infection. The study recorded a total infection with Entamoeba spp (26%) by PCR method and result nested multiplex polymerase chain reaction using three primers  ,that show Entamoeba histolytica (26%)only .This is the first report of molecular detection of E. histolytica, E. dispar and E. moshkovskii infection among wild rats in Malaysia. This study provides useful information about the potential risks of zoonotic agents and the importance of developing control measures to prevent zoonotic transmission.