Cytotoxicity potential of local isolates of Lactobacillus Acidophilus Extracts on Colon Cancer Cell Line

Abstract

Lactobacillus acidophilus isolated locally from dairy samples and identified by biochemical and molecular methods bacterial extract were examined to explore potential antitumor activity by apply on colon cancer cell line CaCo-2.The viability results revealed that all strains extract have cytotoxicity effect on colon cancer cell line in ranged between 36-79% at 400 µg/ml,and more affected one  was chosen for more analysis by high content screening assays that show the extract decreased significantly the viability in concentrations ranged between 200-25 100µg/ml.mitochondrial membrane potential and cytochrome C releasing increased significantly as results of apoptosis processes ,while membrane permeability did not affected  decreased slightly  result of necrosis process.