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Biologically, the nanoparticles of cobalt synthesized by aqueous leaves extract of Conocarpus erectus L. The cobalt nanoparticles appear rapidly with change in color when the leaf extract mixed with CoCl2.6H2O (0.1mM). The cobalt characterized by UV Visible Absorption Spectrometer (UV-S), X-Ray Diffraction nanoparticles were(XRD), Fourier Transform infrared (FTIR), and Scanning Electron Microscopes (SEM).By using UV-S, the wave length was around 350-400 nm, while the size of cobalt nanoparticles was around 4.9 nm and the size particles of leave extract alone was 17.1 nm,characterized by XRD. The SEM technique represent more spherical aggregation in cobalt nanoparticles then in leaves extract alone. The technique of FTIR showed the presence of active sites for alcoholic, phenolic, amines and other compounds.