Study of Il-28b Gene Polymorphism among Pregnant Women with Chronic Viral Hepatitis B

Abstract

The genetic marker IL-28B, which allows partly to predict the course of the disease and the effectiveness of treatment, polymorphism of the interleukin 28B gene determines to a certain extent the sensitivity of the patient's immune system to the viral load and determines the course and outcome of the disease. Thus, the study of the IL-28B gene polymorphism will provide information on the functional activity of various types of immunocompetent cells; the severity of the inflammatory process, its transition to the systemic level and prognosis; on the ratio of the processes of activation of T-helpers of types 1 and 2, which is very important in the diagnosis and treatment of immunopathological processes. The analysis of genetic studies in a healthy population for two alleles showed that OPN rs12979860 of the IL-28B gene: CC - 38%, CT - 51%, TT - 12%; OPN rs8099917 of the IL-28B gene: TT - 56%, TG - 38%, GG - 7%. Analysis of polymorphism of the IL-28B gene in the group of pregnant CHBV showed that ARF rs12979860 of the IL-28B gene: CC - 32%, CT - 66%, TT - 4%; ARF for rs8099917 of the IL-28B gene: TT - 65%, TG - 25%, GG - not detected (0%). The frequency of occurrence of IL-28B gene haplotypes among healthy individuals and pregnant women with CVHB showed that among healthy individuals in 36% of cases there was a CC / TT haplotype, in 31% of cases - CT / TG, 21% - CT / TT, 7% - TT / TG, 4% - TT / GG, 1% - CT / GG and 0% each - TT / TT, CC / -, CT / -, - / TT. Analysis of haplotypes in groups of pregnant women with hepatitis B showed that in 25.7% of cases the CC / TT haplotype was encountered, in 27% of cases - CT / TG, 31.1% - CT / TT, 1.4% - TT / TG, 2.7% - TT / TT, 5.4% - - / TT, 5.4% - CC / -, 1.4% - CT / - and 0% each - TT / GG and CT / GG.