Antiproliferative and apoptosis inducing activity of PHB (polyhydroxybutyrate) from Bacillus Cereus against HeLa and L929 cell lines

Abstract

            Natural product research has employed well known applications in pharmaceutical industries, human healthcare, nutrition and therapeutic application. Polyhydroxybutyrate (PHB) is a synthesized macromolecule from bacteria that is commonly used due to its rapid degradation in natural environment and their abundant occurrence from natural resource. Our aim is to isolate potential PHB producing bacteria from marine invertebrates and its cytotoxic effect against L929 and HeLa cell lines and apoptotic effects of PHB. Cytotoxic effect of PHB was more effective against HeLa cell lines by inhibiting 48.90% for 100µg.ml^-1 and showed good IC₅₀ value. Apoptotic effect against HeLa cell line revealed late apoptosis by orange-stained nuclei with chromatin condensation. Flow cytometric analysis showed inhibitory effect on cell cycle progression at G0/G1 phase. These findings could open a new possibility to use PHB as potential therapeutics from marine origin to treat cervical cancer.