Antibiotic Susptipility Profile and Virulence Factors Profile of E.Coli Isolated from Otitis Media

Abstract

The current study aimed to find Antibiotic susptipility profile and virulence factors profile of E.coli isolated from otitis media, for this purpose 200 ear swabs collected from patients  suffering from otitis media whom  attending to the ENT consulting clinic at Tikrit Teaching Hospital and the private outpatient clinic in Tikrit city. Bacterial isolation and identification were done, virulence factors profile detected by classical and genetic methods, and Antibiotic susptipility were done.

The result shows that out of 200 ear sample collected from otitis media, E.coli in rate of 10 % (20 out of 200), virulence factor detection by classical methods shows that he results of the current study showed that E.coli possessed protease, lecithinase, Dnase, Hemolysin and urease were 10%, 5%,20%, 20% and 0% respectively, while the enzymes denase and hemolysin appeared by 45.6% and 71.7%, respectively. When using genetic methods, the detection of virulence genes IroN, IutA, and F17 genes by 55%, 70%, and 45%, respectively. The results of the current study showed high resistant rate were for Ampicillin (90%) and   for Cefotaxime and Amoxicillin (55%), while the low resistant is for Impenem (10%) and Ceftazidime (15%).