Influence of Some Phytofractions of Strychnos Nux Vomica on Cutaneous Wound Healing in Albino Rats

Abstract

AIM- Influence of Some Phytofractions of Strychnos Nux Vomica on Cutaneous Wound Healing in Albino Rats.

MATERIAL & METHODS- As per facts, references received from literature survey, the seeds of Strychnos nux-vomica   were selected as plant materials for investigational study. The dried seeds of Strychnos nux-vomica were extracted by successive solvent extraction method with the help of soxhlet apparatus. The treatment was done topically in all the groups.10 The extract of both plants was applied at a dose of 100 mg/kg/day (In the form of 10 % w/w extract ointment in steric acid) for 16 days. Wound areas were measured on days 1, 4, 8 and 16 for all groups, using a transparency sheet and a permanent marker. Recording of wound areas were measured on graph paper. The day of scar falling, after wounding without any residual raw wound was considered as the day of epitheliazation.

RESULTS- The % yield of ethanolic, methanolic, petroleum ether, chloroform and acetone extracts of dried seeds of Strychnos nux-vomica was found 16.55, 4.25, 2.5, 8.50 and 7.90 % respectively. The ethanolic extract of Strychnos nux-vomica seeds showed presence of high amount of phenolics and tannin compounds. In Extract treated groups; ethanolic extract of S. nux-vomica seeds treated rats showed highest wound closure 96.44% after 11 days of continuous treatment with extract ointment, which was more than 79.86% of normal control group respectively. The chloroform extract and methanol extract treated groups also exhibited significant increase in wound closure more than both control groups.The wound area of ethanolic extract treated rats was measured about 10.33 mm2 which was very much less than non treated normal (50.33 mm2) rats. In other treatment groups the chloroform and methanol extract treated rats also showed significant decrease in wound area upto 21.67 and 27.67 mm2 respectively.

CONCLUSION- The ethanolic extract and ethylacetate soluble fraction of ethnaolic extract of seeds of plant Strychnos nux-vomica were found to be effective in management of wound healing complications in normal albino rats.  In excision wound model the extracts and fractions of S. nux-vomica showed decrease in wound area, day of scar falling and increase in % wound closure in treated normal rats as compared to non treated normal rats.