Heritability in Five Hemp Mother Plants on their Progeny from Clonal Tissue under Controlled Greenhouse Conditions

Abstract

The purpose of the research is to evaluate five cannabinoid plants' heritability in the progeny generated from said mothers. The experiment was carried out in Jacksonville, Montevideo, Uruguay, during the agricultural year 2019-2020; for the analysis, forty-seven (47) clones were obtained from the five (05) plants of the cultivar Romalex were evaluated and which were sown at a density of 1.3 plants per m2. In the selection, the clones INVPS00006215 and INVPS00007157 were taken for having the highest quantitatively higher Total percentage values of Cannabidiol CBD and for statistically differentiating from the remaining ten clones of mother 256. The methods applied in this research work are analytical and statistical, corresponding to the experimental type; in the measurement, the High-Pressure Liquid Chromatographer HPLC method was used. The statistical analyzes presented include analysis of variance, Pearson's correlation, and regression. They are taking the information on regression coefficients, and it was interpreted for each unit in which the total CBD is increased, and the Tetrahydrocannabinol THC is increased by 12.81, starting from a minimum CBD was observed that the third week is the best results concerning the total percentage CBD. Plant 256 is the one with the lowest standard deviation, with a value of ± 0.57. Genetic variation (heritability) is 15.04% for CBD trait. For the case of total CBD and THC with a value of r = 0.96, that indicates there is a high and positive quantitative correlation.