In Vitro Anti-Biofilm Efficacy Of Achyranthes Aspera (L) Methanolic Extract Against Urinary Tract Infection Causing Escherichia Coli

Abstract

Urinary track and medical device associated infections caused by biofilm forming Escherichia coli (E. coli) plays a primary role in the pathogenesis of urinary tract infection (UTI). The biofilm formed by these pathogens are highly resistant to conventional antibiotic therapies and they are also protected by host immune response, hence they are poorly responding to therapeutic agents. Different parts of medicinal plants are largely utilized as potential source of bioactive agents to cure many diseases. Based on this context the present study conducted to determine the anti-biofilm activity of the methanolic extract of folkloric medicinal plant Achyranthes aspera (L). The plant extract was subjected to Thin Layer Chromatography (TLC) and column was packed with silica (stationary phase). Totally nine different elusions were collected with the usage of different organic solvents (mobile phase) and TLC was again performed for all fractions. The elusion which shown single band in the Ultra Violet (UV) chamber was selected as a test sample for antibacterial activity.

The Minimum Inhibitory Concentration (MIC) was done for the same, then the least concentration which shown best result was used to check the anti-biofilm activity against UTI causing E. coli. The anti-biofilm activity of the elusion has exhibited above 80 %. From this work we have concluded that this plant is highly capable to kill E. coli biofilm and which can be used to develop anti-biofilm medicines for UTI